Part:BBa_K2637023:Design

BD-KaiC, Gal4 DNA binding domain fused with KaiC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 725
  Illegal BglII site found at 1232
  Illegal XhoI site found at 234
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 153
  

Design Notes

Gal4 DNA binding domain fused with KaiC gene. KaiC has been optimized in yeast.

Source

Gal4 DNA binding domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942.